Technical Data
C2589-57A
CDX2 (Homeobox Protein CDX-2, CDX-3, Caudal-type Homeobox Protein 2, CDX3)
Description:
CDX2 is a caudal-type homeobox domain-containing transcription factor, important in the development and differentiation of the intestines.It is a part of a regulatory network that orchestrates a developmental program of proliferation, morphogenesis, and gene expression in the intestinal epithelium. It has 311aa and its homeodomain is a 60-aamotif containing a sequence-specific DNA binding domain. The human CDX2 gene is localized to chromosome 13q12-13. The protein has been shown to positively regulate genes expressed in the small intestine such as sucraseisomaltase, lactase and phospholipase A. CDX2 has been implicated in disorders involving abnormal intestinal differentiation and neoplasia. It is also a sensitive and specific marker for colorectal adenocarcinoma, although its expression is decreased among higher grade and stage tumors, and it is not invariably present in metastases from positive primaries.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (formalin fixed paraffin embedded): 1:25-1:50
Optimal dilutions to be determined by the researcher.

Positive Control:
Colon cancer

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG111E125Supernatant
SizeStorageShippingSourceHost
500ul-20CBlue IceHumanMouse
Concentration:
Not determined.
Immunogen:
Prokaryotic recombinant corresponding to 180aa N-terminal region of the human CDX2 molecule. Cellular Localization: Nuclear.
Purity:
Supernatant
Form
Supplied as a liquid, 0.05% sodium azide.
Specificity:
Recognizes human CDX2. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bai et al. Cancer Letter 176: 47, 2002 2. Hinoi et al. Am J Pathol 159: 2239, 2001 3. Silberg et al. Gastroenterology 119: 961, 2000 4. Sivagnanasundaram et al. British Journal of Cancer 84: 218, 2001. 5. Drummond et al. Ann Hum Genet 61: 393, 1997. 6. Vassil et al. Modern Pathology 17: 1392, 2004.