Technical Data
C2589-60A
CEACAM 1,3,4,5,6 (Carcinoembryonic Antigen Related Cell Adhesion Molecule)
Description:
CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family. It consists of seven CEACAM (CEACAM 1, CEACAM 3-CEACAM 8) and 11 pregnancy-specific glyco-protein (PSG 1-PSG 11) members. The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leukocytes. Over-expression of CEA/ CEACAM 5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker. The function of CEACAM family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria.

Applications:
Suitable for use in ELISA, Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG16D306Affinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
CEACAM5 partially purified from a perchloric acid extract from liver metastases of colonic tumors.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied a a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity:
Recognizes human CEACAM1, 3, 4, 5 and 6
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. P. Jantscheff, et al.; Biomed. Biochim. Acta 50, 1261 (1991) 2. F. Grunert, et al.; Leukocyte Typing VI: White cell Differentiation Antigens (T. Kishimoto, et al., eds.), Garland Publishing Inc., New York 1012 (1996)3. S.D. Gray-Owen, et al.; EMBO J. 16, 3435 (1997) 4. A. Krop-Watorek, et al.; Cancer Lett. 139, 15 (1999) 5. A. Popp, et al.; Cell. Microbiol. 1, 169 (1999).