		Technical Data
C2589-60A	CEACAM 1,3,4,5,6 (Carcinoembryonic Antigen Related Cell Adhesion Molecule)

Description:
CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family. It consists of seven CEACAM (CEACAM 1, CEACAM 3-CEACAM 8) and 11 pregnancy-specific glyco-protein (PSG 1-PSG 11) members. The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leukocytes. Over-expression of CEA/ CEACAM 5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker. The function of CEACAM family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria.

Applications:
Suitable for use in ELISA, Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	6D306	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		4°C (-20°C Glycerol)	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

CEACAM5 partially purified from a perchloric acid extract from liver metastases of colonic tumors.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied a a liquid in PBS, pH 7.2, 0.01% sodium azide.

Specificity:

Recognizes human CEACAM1, 3, 4, 5 and 6

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. P. Jantscheff, et al.; Biomed. Biochim. Acta 50, 1261 (1991) 2. F. Grunert, et al.; Leukocyte Typing VI: White cell Differentiation Antigens (T. Kishimoto, et al., eds.), Garland Publishing Inc., New York 1012 (1996)3. S.D. Gray-Owen, et al.; EMBO J. 16, 3435 (1997) 4. A. Krop-Watorek, et al.; Cancer Lett. 139, 15 (1999) 5. A. Popp, et al.; Cell. Microbiol. 1, 169 (1999).