		Technical Data
C2590-10	CDYL (DKFZP586C1622, chromodomain protein, Y chromosome-like, CDY-like, autosomal, testis-specific chromodomain Y-like protein)

Description:
Chromodomain Y is a primate-specific Y-chromosomal gene family expressed exclusively in the testis and implicated in infertility. Although the Y-linked genes are testis-specific, this autosomal gene is ubiquitously expressed. The Y-linked genes arose by retrotransposition of an mRNA from this gene, followed by amplification of the retroposed gene. Proteins encoded by this gene superfamily possess a chromodomain, a motif implicated in chromatin binding and gene suppression, and a catalytic domain believed to be involved in histone acetylation. Multiple proteins are encoded by transcript variants of this gene.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:32,000 (detection limit dilution; peptide coated on plate)
Western Blot: 0.1-0.3ug/ml. Approximately 35kDa
band observed in human kidney lysates (calculated MW of 31.1kDa according to
NP_036269.1). The observed molecular weight corresponds to earlier findings in
literature (Pullamsetti et al).
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Goat

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide C-SMLKYLQRKIDEF, representing the C-Terminus of the human protein according to NP_004815; NP_736607; NP_736608.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in Tris-saline, pH 7.3, containing 0.5% BSA and 0.02% sodium azide.

Specificity:

Recognizes human CDYL . Species sequence homology: mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Lahn BT, Page DC. Retroposition of autosomal mRNA yielded testis-specific gene family on human Y chromosome. 2. Nat Genet. 1999 Apr;21(4):429-33. Erratum in: Nat Genet 1999 Jun;22(2):209. PMID: 10192397. 3. Pullamsetti et al, FASEB J. 2005 Jul;19(9):1175-1177.