Technical Data
C2590-10
CDYL (DKFZP586C1622, chromodomain protein, Y chromosome-like, CDY-like, autosomal, testis-specific chromodomain Y-like protein)
Description:
Chromodomain Y is a primate-specific Y-chromosomal gene family expressed exclusively in the testis and implicated in infertility. Although the Y-linked genes are testis-specific, this autosomal gene is ubiquitously expressed. The Y-linked genes arose by retrotransposition of an mRNA from this gene, followed by amplification of the retroposed gene. Proteins encoded by this gene superfamily possess a chromodomain, a motif implicated in chromatin binding and gene suppression, and a catalytic domain believed to be involved in histone acetylation. Multiple proteins are encoded by transcript variants of this gene.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:32,000 (detection limit dilution; peptide coated on plate)
Western Blot: 0.1-0.3ug/ml. Approximately 35kDa
band observed in human kidney lysates (calculated MW of 31.1kDa according to
NP_036269.1). The observed molecular weight corresponds to earlier findings in
literature (Pullamsetti et al).
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide C-SMLKYLQRKIDEF, representing the C-Terminus of the human protein according to NP_004815; NP_736607; NP_736608.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris-saline, pH 7.3, containing 0.5% BSA and 0.02% sodium azide.
Specificity:
Recognizes human CDYL . Species sequence homology: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Lahn BT, Page DC. Retroposition of autosomal mRNA yielded testis-specific gene family on human Y chromosome. 2. Nat Genet. 1999 Apr;21(4):429-33. Erratum in: Nat Genet 1999 Jun;22(2):209. PMID: 10192397. 3. Pullamsetti et al, FASEB J. 2005 Jul;19(9):1175-1177.