Technical Data
C2605-54A
CDC6, phosphorylated Ser54 (Cell Division Control Protein 6 Homolog, CDC6-related Protein, p62(cdc6), HsCDC6, Cdc18-related Protein, HsCDC18, CDC18L)
Description:
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins.

Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser54 of human Cdc6 (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human Cdc6 phosphorylated at Ser55.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Alexandrow, M.G., et al., Mol. Cell. Biol. 24(4):1614-1627 (2004). 2. Yim, H., et al., Mol. Biol. Cell 14(10):4250-4259 (2003). 3. Clay-Farrace, L., et al., EMBO J. 22(3):704-712 (2003). 4. Pelizon, C., et al., EMBO Rep. 3(8):780-784 (2002). 5. Bermejo, R., et al., Mol. Biol. Cell 13(11):3989-4000 (2002).