Technical Data
C2615-02B
CENP-A, phosphorylated (Ser7) (Centromere Potein A)
Description:
Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Immunoblot Analysis: 0.25-1ug/ml of this lot detected phosphorylated CENP-A (Ser7) in acid extracted proteins from colcemid-arrested HeLa cells.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ug-20°CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
KLH-conjugated, synthetic peptide contianing the sequence ..RRpSRK… in which pS is phosphoserine corresponding to aa7 of human CENP-A
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
Specificity:
Recognizes phosphorylated human CENP-A (Ser7), Mr ~17kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Zeitlin, S.G., et al., J. Cell Sci. (114): 653-661, 2001. 2. Zeitlin, S.G., et al., J. Cell Biol. (155): 1147-1157, 2001.