Technical Data
C2615-02C
CENP-A, CT (Centromere Potein A)
Description:
Centromere protein A (CENP-A) is a 17kD centromere-specific histone variant with 62% amino acids homology to the C-terminal of histone H3. Localized in the centromere, it plays a central role in the centromere-specific chromatin formation. The depletion of histone H3 at the CENP-A binding domain suggests CENP-A to be a possible replacement for histone H3 in the packaging process of a-satellite DNA into primary chromation structure (2). CENP-A is essential in the formation of specialized nucleosomes at the centromere, implicating CENP-A as a centromere-specific epigenetic marker (3).

Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:80
Western Blotting: 1:20,000
Immunohistochemistry: 1:250-500
Immunocytochemistry: 1:250-500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG6k123Supernatant
SizeStorageShippingSourceHost
100ul4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to residues in the C-term of human CENP-A
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-Glcine (pH 7.4), 0.15M NaCl, 40% glycerol, 0.01% sodium azide and 0.05% BSA.
Specificity:
Recognizes human CENP-A, CT (Centromere Potein A) Species Crossreactivity: Rat. No cossreactivity with mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Warburton PE, Cooke CA, et al. Curr Biol 7:901-904, 1997.
2.Yokda K, et al. Proc Natl Acad Sci USA 97:7266-7271, 2000.
3.Choo KHA, et al. Cell Biol 10:182188, 2000.