Technical Data
C2615-02C
CENP-A, CT (Centromere Potein A)
Description:
Centromere protein A (CENP-A) is a 17kD centromere-specific histone variant with 62% amino acids homology to the C-terminal of histone H3. Localized in the centromere, it plays a central role in the centromere-specific chromatin formation. The depletion of histone H3 at the CENP-A binding domain suggests CENP-A to be a possible replacement for histone H3 in the packaging process of a-satellite DNA into primary chromation structure (2). CENP-A is essential in the formation of specialized nucleosomes at the centromere, implicating CENP-A as a centromere-specific epigenetic marker (3).

Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:80
Western Blotting: 1:20,000
Immunohistochemistry: 1:250-500
Immunocytochemistry: 1:250-500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG6k123Supernatant
SizeStorageShippingSourceHost
100ul4°C (-20°C Glycerol)Blue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to residues in the C-term of human CENP-A
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-Glcine (pH 7.4), 0.15M NaCl, 40% glycerol, 0.01% sodium azide and 0.05% BSA.
Specificity:
Recognizes human CENP-A, CT (Centromere Potein A) Species Crossreactivity: Rat. No cossreactivity with mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Warburton PE, Cooke CA, et al. Curr Biol 7:901-904, 1997.
2.Yokda K, et al. Proc Natl Acad Sci USA 97:7266-7271, 2000.
3.Choo KHA, et al. Cell Biol 10:182–188, 2000.