Technical Data
C2615-10
CENP-A (Centromere Protein A)
Description:
Centromere protein A (CENP-A) is a 17-19kD centromere-specific histone variant that is 62% identical to the carboxy-terminal domain of histone H3. In the presence of DNA CENP-A forms an octameric complex with histones H4, H2A, and H2B. CENP-A defines active centromere regions by forming centromere-specific nucleosomes on which kinetochores are assembled. CENP-A is specifically located to the inner plate of the kinetochore and it is essential for kinetochore targeting of CENP-C and other kinetochore components. CENP-A is required for nucleosomal packaging of centromeric DNA at interphase.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1ug/ml
Immunohistochemistry: 5ug/ml
Immunocytochemistry: 10ug/ml
Optimal dilutions to be determined by researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG14E43Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to a portion of human CENP-A.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 50% glycerol.
Specificity:
Recognizes human CENP-A (~18kD).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ando, S., et al (2002). Mol. Cell. Biol. 22, 2229-2241.2. Sullivan K.F. et al., J cell bIol 127:581-592 (1994) 3. Pusarla R-H FEBS J. 272: 5149-5168 (2005)