		Technical Data
C2615-10	CENP-A (Centromere Protein A)

Description:
Centromere protein A (CENP-A) is a 17-19kD centromere-specific histone variant that is 62% identical to the carboxy-terminal domain of histone H3. In the presence of DNA CENP-A forms an octameric complex with histones H4, H2A, and H2B. CENP-A defines active centromere regions by forming centromere-specific nucleosomes on which kinetochores are assembled. CENP-A is specifically located to the inner plate of the kinetochore and it is essential for kinetochore targeting of CENP-C and other kinetochore components. CENP-A is required for nucleosomal packaging of centromeric DNA at interphase.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1ug/ml
Immunohistochemistry: 5ug/ml
Immunocytochemistry: 10ug/ml
Optimal dilutions to be determined by researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	4E43	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Synthetic peptide corresponding to a portion of human CENP-A.

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2, 50% glycerol.

Specificity:

Recognizes human CENP-A (~18kD).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Ando, S., et al (2002). Mol. Cell. Biol. 22, 2229-2241.2. Sullivan K.F. et al., J cell bIol 127:581-592 (1994) 3. Pusarla R-H FEBS J. 272: 5149-5168 (2005)