Technical Data
C2615-10A
CENP-A (Centromere Protein A, Histone H3-like Centromeric Protein A, Centromere Autoantigen A)
Description:
Centromere protein A (CENP-A) is a 17kD centromere-specific histone variant with 62% amino acids homology to the C-terminal of histone H3. Localized in the centromere, it plays a central role in the centromere-specific chromatin formation. The depletion of histone H3 at the CENP-A binding domain suggests CENP-A to be a possible replacement for histone H3 in the packaging process of -satellite DNA into primary chromatin structure. CENP-A is essential in the formation of specialized nucleosomes at the centromere, implicating CENP-A as a centromere-specific epigenetic marker.

Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:80
Western Blot: 1:5000
Immunohistochemistry: 1:250-1:500
Immunocytochemistry: 1:250-1:500
Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa Whole Cell lysate.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG9H220Supernatant
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to the C-terminal region of CENP-A, conjugated to KLH. Cellular Localization: Nucleus. Centromere. Kinetochore.
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
Specificity:
Recognizes the C-terminal region of human CENP-A. Species Crossreactivity: rat. Does not react with mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Durand-Dubief M, Ekwall K. (2008). Bioessays. 30(6):526-9 2. Black BE, Bassett EA. (2008). Curr Opin Cell Biol. 20(1):91-100 3. Palmer DK, et al. (1987). J Cell Biol. 104(4):805-15.