		Technical Data
C2615-10A	CENP-A (Centromere Protein A, Histone H3-like Centromeric Protein A, Centromere Autoantigen A)

Description:
Centromere protein A (CENP-A) is a 17kD centromere-specific histone variant with 62% amino acids homology to the C-terminal of histone H3. Localized in the centromere, it plays a central role in the centromere-specific chromatin formation. The depletion of histone H3 at the CENP-A binding domain suggests CENP-A to be a possible replacement for histone H3 in the packaging process of -satellite DNA into primary chromatin structure. CENP-A is essential in the formation of specialized nucleosomes at the centromere, implicating CENP-A as a centromere-specific epigenetic marker.

Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:80
Western Blot: 1:5000
Immunohistochemistry: 1:250-1:500
Immunocytochemistry: 1:250-1:500
Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa Whole Cell lysate.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG	9H220	Supernatant

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

As reported

Immunogen:

Synthetic peptide corresponding to the C-terminal region of CENP-A, conjugated to KLH. Cellular Localization: Nucleus. Centromere. Kinetochore.

Purity:

Supernatant

Form

Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.

Specificity:

Recognizes the C-terminal region of human CENP-A. Species Crossreactivity: rat. Does not react with mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Durand-Dubief M, Ekwall K. (2008). Bioessays. 30(6):526-9 2. Black BE, Bassett EA. (2008). Curr Opin Cell Biol. 20(1):91-100 3. Palmer DK, et al. (1987). J Cell Biol. 104(4):805-15.