Technical Data
C2615-20
CENP-F (Centromere protein F , CENPF, centromere protein F (350/400kD, mitosin), CENF, PRO1779, mitosin, AH antigen, CENP-F kinetochore protein, centromere protein F (400kD), cell-cycle-dependent 350K nuclear protein)
Description:
The kinetophore is a macromolecular structure that is associated with the centromeres of chromosomes and is responsible for establishing and maintaining the connections with the microtubules of the mitotic spindle. The proteins that reside within the chromosomal domain fall into two broad classes: those that associate with the centromere throughout the cell cycle and those that are found at the centromere in a cell cycle specific manner. Members of the first class include CENP-A, CENP-B, and CENP-C. The members of the second class include several ubiquitous cellular proteins such as calmodulin, tubulin, dynein and p34 cdc2 and also CENP-E and CENP-F. CENP-F appears to be the earliest member to interact with the centromere-kinetochore complex. CENP-F also shows a cell-cycle distribution that is both temporally regulated and diverse in terms of the structural components it localizes to and therefore may play a role in several mitotic events. (1-3)

Cellular Localization:
As cells progress through S phase, weak nuclear staining is observed. In G2 phase, very bright CENP-F staining is detected uniformly throughout the nucleus but excluded from the nucleoli.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ul-20CBlue IceHumanRabbit
Concentration:
~1 mg/ml
Immunogen:
A bacterial fusion protein from the C-terminus of CENP-F.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 50% glycerol, 0.1% sodium azide.
Specificity:
Specific for human CENP-F.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. H. Liao et al, CENP-F Is a Protein of the Nuclear Matrix That Assembles onto Kinetochores at Late G2 and Is Rapidly Degraded After Mitosis. The Journal of Cell Biology, 130(3): 507-518, 1995. 2. J.B. Rattner et al, CENP-F is an ca. 400 kDa Kinetochore Protein that Exhibits a Cell-Cycle Dependent Localization. Cell Motility and the Cytoskeleton, 26: 214-226, 1993. 3. S-C Lu et al. Markers of Cell Proliferation in Normal Epithelia and Dysplastic Leukoplakias of the Oral Cavity. Cancer Epidemiology, Biomarkers and Prevention. 7: 597-603, 1998