Technical Data
C2615-21A2
CENP-F (Centromere protein F , CENPF, centromere protein F (350/400kD, mitosin), CENF, PRO1779, mitosin, AH antigen, CENP-F kinetochore protein, centromere protein F (400kD), cell-cycle-dependent 350K nuclear protein)
Description:
Clone 1D8 recognizes mitosin, a 350kD phosphoprotein, that is specifically involved in mitotic-phase progression. Mitosin is expressed throughout S, G2 , and M phases of the cell cycle but is absent G0, and G1. It associates with the mitotic apparatus during M-phase, and is redistributed from the nucleus to the centromere, spindle, and midbody during M-phase progression. Its usefulness as a proliferation marker is under active investigation.

Positive Control: Human tissue containing proliferating cells (e.g., germinal centers of lymph nodes).

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a,k8J1313Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHuman Mouse
Concentration:
As reported
Immunogen:
Fusion protein expressed in E. coli corresponding to amino acids 1759-2093.
Purity:
Purified by Protein A affinity chromatography.
Form
As reported
Specificity:
Reacts with human Mitosin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Allred, D.C., et al. (1997). Mitosin (a new proliferation marker) correlates with clinical outcome in node negative breast cancer. Lab. Invest. 15A(69).
2.Zhu X., et al. (1995). Characterization of a novel 350-kilodalton nuclear phosphoprotein that is specifically involved in mitotic-phase progression. Mol. Cell. Biol. 15: 5017-5029.
3.Clark,G. et al. Mitosin (a new proliferation marker) correlates with clinical outcome in node-negative breast cancer. Cancer Research. 57: 5505-5508, 1997.