		Technical Data
C2615-24B	CENP-A (Centromere Protein A)

Description:
Centromere protein A (CENP-A) is a centromere-specific histone variant which forms an octameric complex with Histones H4, H2A, and H2B, in the presence of DNA. CENP-A defines active centromere regions by forming centromere-specific nucleosomes on which kinetochores are assembled. CENP-A is specifically located to the inner plate of the kinetochore and it is essential for kinetochore targeting of CENP-C and other kinetochore components. CENP-A is required for nucleosomal packaging of centromeric DNA at interphase.

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 5ug/ml
Western Blot: 1ug/ml
Immunocytochemistry: 10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	8J986	Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		4°C (-20°C Glycerol)	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Synthetic peptide cooresponding to aa 3-19, PRRRSRKPEAPRRRSPS, of human CENP A. Cellular Localization: Nuclear.

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2, 50% glycerol. No preservatives added.

Specificity:

Recognizes human CENP A. at ~18kD.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Ando S et al. CENP-A, -B, and -C chromatin complex that contains the I-type alpha-satellite array constitutes the prekinetochore in HeLa cells. Mol Cell Biol 22:2229-41 (2002).