Technical Data
C2615-24J
CENP-J (Centromere Protein J, CENPJ, BM032, Centrosomal P4.1-associated Protein, CPAP, LAG-3-associated Protein, LAP, LYST-interacting Protein 1, LIP1, MCPH6, MGC131581, MGC131582, MGC142222, MGC142224) (Azide Free)
Description:
This gene encodes a protein that belongs to the centromere protein family. During cell division, this protein plays a structural role in the maintenance of centrosome integrity and normal spindle morphology, and it is involved in microtubule disassembly at the centrosome. This protein can function as a transcriptional coactivator in the Stat5 signaling pathway, and also as a coactivator of NF-kappaB-mediated transcription, likely via its interaction with the coactivator p300/CREB-binding protein. Mutations in this gene are associated with primary autosomal recessive microcephaly, a disorder characterized by severely reduced brain size and mental retardation.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (paraffin): 1.5ug/ml
ELISA: 1ug/ml-3ng/ml
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a10B2207Affinity Purified
SizeStorageShippingSourceHost
100ul4C/-20C (No Glycerol)Blue IceHumanMouse
Concentration:
As reported
Immunogen:
Recombinant protein corresponding to a portion of amino acids within aa979-1089 of human CENPJ.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added (Azide free).
Specificity:
Recognizes human CENPJ.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.