Technical Data
CENP-Q (Centromere Protein Q)
Cenp-Q (also known as centromere protein Q or CENPQ) is a nuclear/centromeric protein that is one of the critical components that constitutes the CENP-O complex at the kinetochores and appears to stabilize PBIP1/CENP-U(50)/MLF1IP in the complex. This complex is important for proper recruitment of polo-like kinase 1 (Plk1) to the mitotic kinetochores. A failure in this process results in improper microtubule attachment to the kinetochores and chromosome missegregation that ultimately lead to aneuploidy.

Suitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:100-1:500
ELISA: 1:20,0001:30,000
Western Blot: 1:2,000-1:2,500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
As reported
Full-length human CENP-Q recombinant protein.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Species Sequence Homology: macaque(91%), horse(76%), bovine(74%), dog(73%), swine (71%), mouse(65%) and rat(60%).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Hori T, Okada M, Maenaka K, Fukagawa T. (2008) CENP-O Class Proteins Form a Stable Complex and Are Required for Proper Kinetochore Function. Mol Biol Cell. Mar;19(3):843-54. Epub 2007 Dec 19.
2.Okada M, Cheeseman IM, Hori T, Okawa K, McLeod IX, Yates JR 3rd, Desai A, Fukagawa T. (2006) The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres. Nat Cell Biol. May;8(5):446-57. Epub 2006 Apr 16. 3.Foltz DR, Jansen LE, Black BE, Bailey AO, Yates JR 3rd, Cleveland DW. (2006) The human CENP-A centromeric nucleosome-associated complex. Nat Cell Biol. May;8(5):458-69. Epub 2006 Apr 16. with bisbenzimide. Personal Communication, Kyung S. Lee, CCR-NCI, Bethesda, MD.