Technical Data
C2615-25
CENP-Q (Centromere Protein Q)
Description:
Cenp-Q (also known as centromere protein Q or CENPQ) is a nuclear/centromeric protein that is one of the critical components that constitutes the CENP-O complex at the kinetochores and appears to stabilize PBIP1/CENP-U(50)/MLF1IP in the complex. This complex is important for proper recruitment of polo-like kinase 1 (Plk1) to the mitotic kinetochores. A failure in this process results in improper microtubule attachment to the kinetochores and chromosome missegregation that ultimately lead to aneuploidy.

Applications:
Suitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:100-1:500
ELISA: 1:20,000–1:30,000
Western Blot: 1:2,000-1:2,500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Full-length human CENP-Q recombinant protein.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity:
Species Sequence Homology: macaque(91%), horse(76%), bovine(74%), dog(73%), swine (71%), mouse(65%) and rat(60%).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Hori T, Okada M, Maenaka K, Fukagawa T. (2008) CENP-O Class Proteins Form a Stable Complex and Are Required for Proper Kinetochore Function. Mol Biol Cell. Mar;19(3):843-54. Epub 2007 Dec 19.
2.Okada M, Cheeseman IM, Hori T, Okawa K, McLeod IX, Yates JR 3rd, Desai A, Fukagawa T. (2006) The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres. Nat Cell Biol. May;8(5):446-57. Epub 2006 Apr 16. 3.Foltz DR, Jansen LE, Black BE, Bailey AO, Yates JR 3rd, Cleveland DW. (2006) The human CENP-A centromeric nucleosome-associated complex. Nat Cell Biol. May;8(5):458-69. Epub 2006 Apr 16. with bisbenzimide. Personal Communication, Kyung S. Lee, CCR-NCI, Bethesda, MD.