Technical Data
Centaurin beta 2, CT (ACAP2, Ankyrin Repeat, ARFGAP with Coiled-coil ANK Repeat and PH Domains 2, CENTB2, Cnt b2, KIAA0041, Pleckstrin Homology Domains 2)
Centaurin beta 1 and Centaurin beta 2 are recruited to platelet-derived growth factor-induced dorsal membrane ruffles in NIH 3T3 mouse fibroblasts, and overexpression inhibited ruffle formation. In vitro, Centaurin beta 1 and Centaurin beta 2 preferred ARF6 as substrate rather than ARF1 or ARF5, and the GAP activity of both Centaurins was dependent upon phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). The isolated PH domain of mouse Centaurin beta 2 exhibits moderate affinity for PtdIns(3,5)P2, but it does not bind any other phosphoinositides tested, including PtdIns(4,5)P2. Mutation of a highly conserved arginine in both Centaurins result in lack of ARF-GAP activity. Overexpression in HeLa cells of either Centaurin blocks the formation of ARF6-dependent protrusions. Both Centaurins are also recruited to peripheral, tubular membranes, where activation of ARF6 occurs and allows membrane recycling back to the plasma membrane.

Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:32,000
Western Blot: 1:32,000 & 1-3ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabAffinity Purified
50ug-20CBlue IceHumanGoat
Synthetic peptide corresponding to C-EKLNRFQQDSQKF from human CENTB2 at C-terminal.
Purified by immunoaffinity chromatography.
Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Recognizes human CENTB2. Species sequence homology: Mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.