		Technical Data
C2617-02D3	Centaurin beta 2 (ACAP2, Ankyrin Repeat, ARFGAP with Coiled-coil ANK Repeat and PH Domains 2, CENTB2, Cnt b2, KIAA0041, Pleckstrin Homology Domains 2)

Description:
ACAP2 is a transport carrier belonging to the Centaurin family with three ANK repeats, an Arf-GAP domain, a BAR domain, and a PH domain. ACAP2, a GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) has robust constitutive ARF6 GAP activity, where the GAP activity is stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidic acid but has little activity toward ARF1 and is known to control the return of ARF to the inactive GDB-bound shots. ACAP2, along with ACAP1, is targeted by phosphatidyl inositol (3, 4, 3) triphosphate inhibiting the formation of PDGF-induced dorsal membrane ruffles in NIH3T3 fibroblasts when overexpressed. ACAP2 is phosphorylated upon DNA damage, probably by ATM or ATR. Expression is wide-spread with the highest level in lung.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.25-0.5ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Testis

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to aa350-400 of human ACAP2.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

Specificity:

Recognizes human ACAP2. Species Crossreactivity: chimpanzee, canine, equine, monkey, mouse, rat. Species sequence homology: xenopus, chicken, opossum.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Dowler, S. et al. Biochem. J. 351:19-31 (2000). 2. Jackson, TR. et al. J. Cell Biol. 151:627-638 (2000). 3. Nomura, N. et al. DNA Res. 1:223-229 (1994).