Technical Data
C2617-02D3
Centaurin beta 2 (ACAP2, Ankyrin Repeat, ARFGAP with Coiled-coil ANK Repeat and PH Domains 2, CENTB2, Cnt b2, KIAA0041, Pleckstrin Homology Domains 2)
Description:
ACAP2 is a transport carrier belonging to the Centaurin family with three ANK repeats, an Arf-GAP domain, a BAR domain, and a PH domain. ACAP2, a GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) has robust constitutive ARF6 GAP activity, where the GAP activity is stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidic acid but has little activity toward ARF1 and is known to control the return of ARF to the inactive GDB-bound shots. ACAP2, along with ACAP1, is targeted by phosphatidyl inositol (3, 4, 3) triphosphate inhibiting the formation of PDGF-induced dorsal membrane ruffles in NIH3T3 fibroblasts when overexpressed. ACAP2 is phosphorylated upon DNA damage, probably by ATM or ATR. Expression is wide-spread with the highest level in lung.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.25-0.5ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Testis

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa350-400 of human ACAP2.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity:
Recognizes human ACAP2. Species Crossreactivity: chimpanzee, canine, equine, monkey, mouse, rat. Species sequence homology: xenopus, chicken, opossum.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Dowler, S. et al. Biochem. J. 351:19-31 (2000). 2. Jackson, TR. et al. J. Cell Biol. 151:627-638 (2000). 3. Nomura, N. et al. DNA Res. 1:223-229 (1994).