		Technical Data
C2617-02D5	ACAP2 (Arf-GAP with Coiled-coil, ANK Repeat and PH Domain-containing Protein 2, Centaurin-beta-2, Cnt-b2, CENTB2, KIAA0041)

Description:
GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6).

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry Other applications not tested.

Recommended Dilution:
ELISA: 1:32,000
Western Blot: 1-3ug/ml, observed in human T cell line MOLT4 lysates on ~90kD bands
Immunohistochemistry (Paraffin): 5-10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Goat

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to C-EKLNRFQQDSQKF, from human ACAP2, at C-terminal (NP_036419.3).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

Specificity:

Recognizes human ACAP2. Species sequence homology: Canine, mouse, porcine and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Jackson TR, Brown FD, Nie Z, Miura K, Foroni L, Sun J, Hsu VW, Donaldson JG, Randazzo PA. ACAPs are arf6 GTPase-activating proteins that function in the cell periphery. J Cell Biol. 2000 Oct 30;151(3):627-38.