Technical Data
C2900-04
Ceruloplasmin (Cp, Ferroxidase, Iron (II) Oxygen Oxidoreductase)
Description:
Ceruloplasmin (Cp) is a plasma metalloprotein synthesized and secreted by the liver. Cp (also known as ferroxidase; iron (II):oxygen oxidoreductase, EC 1.16.3.1) is a blue alpha-2-glycoprotein that binds 90 to 95% of plasma copper and has 6 or 7 cupric ions per molecule. It is involved in peroxidation of Fe(II) transferrin to form Fe(III) transferrin. Human Cp is a single polypeptide chain (1065 aa, 135kD; chromosome 8q21.13-q23.1) structurally similar to factors V and VIII. Cp also has abundant expression in specific populations of glial cells within the brain. Hereditary Cp deficiency have profound iron accumulation in most tissues. Cp -/- mice showed no abnormalities in cellular iron uptake but a striking impairment in the movement of iron out of reticuloendothelial cells and hepatocytes. Cp may play an essential physiologic role in determining the rate of iron efflux from cells with mobilizable iron stores.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 1:100,000; using 50-100ng control antigen/well.
Optimal dilutions to be determined by the researcher.

Postive Control:
I1903-25Z8: IgG, Goat, Non-Immune Control

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceGoat
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
Purified bovine pancreatic DNase 1.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 0.1% BSA. Reconstitute in 100ul sterile PBS.
Specificity:
Recognizes Ceruloplasmin at ~135kD. Species Crossreactivity: Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Koschinsky ML et al (1986) PNAS 83, 5086-5090. 2. Daimon M et al (1995) BBRC 208, 1028-1035. 3. Yang F et al (1986) PNAS 83, 3257-3261. 4. Yakahashi N et al (1984) PNAS 81, 390-394. 6. YANG Fm et al (1990) JBC 265, 10780-10785.