Technical Data
C2900-07B
Ceruloplasmin (CP, EC 1.16.3.1, Ferroxidase) (Biotin)
Description:
Ceruloplasmin (Cp) is a plasma metalloprotein synthesized and secreted by the liver. Cp (also known as ferroxidase; iron (II):oxygen oxidoreductase, EC 1.16.3.1) is a blue alpha-2-glycoprotein that binds 90 to 95% of plasma copper and has 6 or 7 cupric ions per molecule. It is involved in peroxidation of Fe(II) transferrin to form Fe(III) transferrin. Cp also has abundant expression in specific populations of glial cells within the brain. Hereditary Cp deficiency have profound iron accumulation in most tissues. Cp -/- mice showed no abnormalities in cellular iron uptake but a striking impairment in the movement of iron out of reticuloendothelial cells and hepatocytes. Cp may play an essential physiologic role in determining the rate of iron efflux from cells with mobilizable iron stores.

Applications:
Suitable for use in ELISA, Western Blot, Immunoelectrophoresis, Single Radial Immunodiffusion and Inhibition. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGHighly Purified
SizeStorageShippingSourceHost
1ml-20CBlue IceRabbit
Concentration:
~10mg/ml (after reconstitution)
Immunogen:
Ceruloplasmin isolated and purified from human serum
Purity:
Purified by ammonium sulfate precipitation and Ion Exchange chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.2. Labeled with Biotin. Reconstitute in 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
Specificity:
Recognizes ceruloplasmin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.