Technical Data
CES1 (Carboxylesterase 1, Acyl Coenzyme A:Cholesterol Acyltransferase, ACAT, Brain Carboxylesterase hBr1, CEH, Egasyn, HMSE, HMSE1, Liver Carboxylesterase 1, MGC117365, Monocyte/macrophage Serine Esterase 1, PCE-1, Serine Esterase 1, SES1, Triacylglycerol
Carboxylesterase 1 is a member of a large family of carboxylesterases that are responsible for the hydrolysis of ester and amide bonds (1, 2). They have broad substrate specificity ranging from small molecule esters such as phenylester to long chain fatty acid esters and thioesters. They play a major role as determinants of pharmacokinetic behavior for most therapeutic agents containing an ester. By de-esterification, they can activate or inactivate the agents. They also participate in detoxification of drugs such as cocaine and heroin in serum and liver. The resulting de-esterified metabolites are secreted out in urine. They can also detoxify organophosphate and carbamate analogues used in agrochemicals or chemical nerve agents, such as malathion, sarin, tabun, and VX. In addition to the hydrolytic activity, they can perform transesterification, a reaction important for cholesterol homeostasis. Carboxylesterase deficiency may be associated with non-Hodgkin lymphoma or B-cell lymphocytic leukemia. CES-1 shares the serine hydrolase fold observed in other esterases (3). CES-1 possesses an endoplasmic reticulum retention signal (HIEL) at its C-terminus.

Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 2ug/ml
Immunoprecipitation: 25ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG2B11n45Affinity Purified
100ug-20CBlue IceHumanMouse
Not determined
Recombinant corresponding to aa19-563 from human CES-1 expressed in NS0 cells (P23141).
Purified by Protein A/G affinity chromatography.
Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
Recognizes human CES-1. Species Crossreactivity: recombinant mouse CES-3. No cross-reactivity with rmCES-2, rmCES-5, or recombinant human CES-2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. 2. 3. Redinbo, M.R. and P.M. Potter (2005) Drug Discovery Today 10:313. Satoh, T. and M. Hosokawa (2006) Chem. Biol. Interactions 162:195. Fleming, C.D. et al. (2007) Biochemistry 46:5603.