Technical Data
CES2 (Cocaine Esterase, Carboxylesterase 2, CE-2, hCE-2, Methylumbelliferyl-acetate Deacetylase 2, ICE)
Carboxylesterase 2 is a member of a serine esterase family composed of enzymes which hydrolyze ester and amide bonds. The members in this family share the serine hydrolase fold observed in other esterases. They have broad substrate specificity from small molecule esters such as phenylester to long-chain fatty acid esters and thioesters. They play a major role in the pharmacokinetics of most therapeutic agents containing an ester. By de-esterification, they can activate or inactivate the agents. They also participate in the detoxification of drugs such as cocaine and heroin in serum and liver. In addition to narcotics, they can also detoxify organophosphate and carbamate analogues used in agrochemicals or chemical nerve agents, such as malathion, sarin, tabun, and VX. In addition to the hydrolytic activity, they can perform transesterification. This reaction is important for cholesterol homeostasis. Three major human CESs have been identified. CES1 is highly expressed in liver. CES2 is present in the small intestine, colon, kidney, liver, heart, brain, and testis. CES3 is brain-specific. Carboxylesterase deficiency may be associated with non-Hodgkin lymphoma or B-cell lymphocytic leukemia.

Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 2ug/ml
Immunocytochemistry: 8-25ug/ml
Immunoprecipitation: 25ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG112K61Affinity Purified
100ug-20CBlue IceHumanMouse
~0.5mg/ml after reconstitution
Recombinant corresponding to aa27-559 from human CES2 expressed in NSO cell line.
Purified by Protein A/G affinity chromatography.
Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
Recognizes human CES2. Does not react with recombinant mouse Carboxylesterase 2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Redinbo, M. R. and P.M. Potter. (2005) Drug Discovery Today, 10:313. 2. Satoh, T. and M. Hosokawa. (2006) Chem.-Biol. Interactions, 162:195. 3.Fleming, C. D. et al. (2007) Biochemistry 46:5603. 4. Imai, T. (2006) Drug Metab. Pharmacokinet. 21:173.