Technical Data
Media
Cetrimide Agar, Non-USP (Pseudosel Agar®) (Powder)
C3100
Microbiological Grade
Storage 4°C    Shipping RT
Components shown as g/liter
Pancreatic Digest of Gelatin 20.0
Magnesium Chloride 1.4
Potassium Sulfate 10.0
Cetrimide 0.3
Agar 15.0
Total46.7g/Liter
Selective isolation of Pseudomonas aeruginosa and other gram-negative, non-fermentative bacteria.

Centrimide Agar is based on Tech Agar, which was developed by King, Ward and Raney. Centrimide is prepared according to this formula with the addition of Centrimide (cetyltrimethylammonium bromide). Cetrimide is a quaternary ammonium, cationic detergent with limited disinfectant action. It causes nitrogen and phosphorus to be released from the bacterial ceII. This germicidal activity is effective on most microorganisms except Pseudomonas aeruginosa. Centrimide Agar Base is supplemented with 1% Glycerol as a source of carbon.

Storage:
Store the sealed bottle containing the dehydrated medium at 4°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige color.


Directions: 1. Dissolve 46.7grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized.

2. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi). 3. Cool to 45.0°C and pour into sterile Petri dishesStorage:Store powdered media at 4°C. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

Microbiological Analysis: Escherichia coli ATCC 25922

Appearance: Light beige, homogeneous, free flowing powder.

Solubility: Light to medium beige, slightly turbid.

pH (25°C): 7.2±0.2


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.