Technical Data
Media
Cetrimide Agar, Non-USP (Pseudosel Agar) (Powder)
C3100
Microbiological Grade
Storage 4C    Shipping RT
Components shown as g/liter
Pancreatic Digest of Gelatin 20.0
Magnesium Chloride 1.4
Potassium Sulfate 10.0
Cetrimide 0.3
Agar 15.0
Total46.7g/Liter
Selective isolation of Pseudomonas aeruginosa and other gram-negative, non-fermentative bacteria.

Centrimide Agar is based on Tech Agar, which was developed by King, Ward and Raney. Centrimide is prepared according to this formula with the addition of Centrimide (cetyltrimethylammonium bromide). Cetrimide is a quaternary ammonium, cationic detergent with limited disinfectant action. It causes nitrogen and phosphorus to be released from the bacterial ceII. This germicidal activity is effective on most microorganisms except Pseudomonas aeruginosa. Centrimide Agar Base is supplemented with 1% Glycerol as a source of carbon.

Appearance:
Beige, homogeneous, free flowing powder.

Solubility:
Light to medium beige, slightly turbid.

pH (25C):
7.20.2

Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.


Directions: Dissolve 46.7g per liter of ddH2O, heating with stirring until completely solubilized. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi). Cool to 45.0C and pour into sterile Petri dishes.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Microbiological Analysis: Escherichia coli ATCC 8739

Appearance: Beige, homogeneous, free flowing powder.

Solubility: Light to medium beige, slightly turbid.

pH (25C): 7.20.2


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.