Technical Data
C3470
Chaperonin 10 (Cpn10, Heat Shock Protein 10, HSP10)
Description:
Chaperonin 10 (Cpn 10 or Hsp10), is the ~10kD mammalian equivalent to GroES of E. coli. Chaperonins play fundamental roles in the folding, assembly, and translocation of other proteins. Chaperonin function in vivo requires two distinct family members, cpn60 and cpn10, both of which are also present in chloroplasts and mitochondria. The best studied cpn60 and cpn10, are the GroEL and GroES proteins of E. coli, respectively. Cpn10 and Cpn60 both exhibit sevenfold axis of symmetry and function as a team in the protein folding and assembly processes. Chaperonin 10 has been located in human platelets but more importantly it may also be present in human maternal serum. It has been reported that human cpn10 is identical with early pregnancy factor (EPF), which is involved in control over cell growth and development. This identification suggests that cpn10 may act like a hormone in stressful situations such as pregnancy.

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot (Colorimetric): 1:1000
Western Blot (ECL): 1:1000-1:2500
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.

Positive Controls:
Heat Shocked HeLa Cell Lysate,
C3450 Chaperonin 10, Recombinant, Human (cpn10, Heat Shock Protein 10, Hsp10)

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4C (-20C Glycerol)Blue IceRatRabbit
Concentration:
Not determined
Immunogen:
An 11 residue synthetic peptide [RDGDILGKYVD] based on the rat cpn10 (residues 91-101) was synthesized and the peptide coupled to KLH. This sequence is identical to human and bovine Cpn10 over these residues.
Purity:
Purified by Protein A affinity chromatography
Form
Supplied as a liquid, in PBS, pH 7.2, 0.09% azide, 50% glycerol
Specificity:
Recognizes a protein, corresponding to the apparent molecular mass of rat Cpn10 on SDS-PAGE immunoblot. at ~10kD. Species Crossreactivity: Human, mouse, bovine, canine, guinea pig, porcine, rabbit, sheep and Xenopus.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hemmingsen, S.M., et al., Nature 333: 330-334 (1988). 2. Gething, M.-J. & Sambrook, J., Nature 355: 33-45 (1992). 3. Gatenby, A.A. & Viitanen, P.V., Annu. Rev. Plant Physiol. Plant Mol. Biol. 45: 469-491 (1994). 4. Morton, H., et al., Nature (London) 249: 459-460 (1974). 5. Cavanagh, A.C. & Morton, H., Eur. J. Biochem. 222: 551-560 (1994). 6. Samali, A., et al., EMBO J. 18: 2040-2048 (1999). 7. Ravagnan, L., et al., Nat. Cell Biol. 3(9): 839-843 (2001). 8. Ruepp, S.U., et al., Toxicol. Sci. 65(1): 135-150 (2002). 9. Schafler, A.E., et al., Ann. Thorac. Surg. 74(3): 767-770 (2002). 10. Wilson-Fritch, L., et al., Mol. Cell Biol. 23(3): 1085-1094 (2003).