Technical Data
C3470A
Chaperonin 10 (Cpn10, Heat Shock Protein 10, HSP10) (Chlamydia)
Description:
Homologs of E. coli GroES make up a family of small heat shock proteins with an approximate molecular mass of 10kD (Hsp10s). Hsp10 acts as a cochaperone and interacts with members of the Hsp60 family (GroEL in E. coli) to promote proper folding of polypeptides. In chlamydia, cpn10 is thought to interact with cHsp60, a reported immunopathological antigen (2), identifying cpn10 as a potential contributor to an immunological response (1).

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry and ELISA. Other applications not tested.

Recommended Dilution:
Western Blot: 0.25ug/ml
Optimal dilutions to be determined by researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a4E44Affinity Purified
SizeStorageShippingSourceHost
25ug4°C (-20°C Glycerol)Blue IceMouse
Concentration:
~0.5mg/ml
Immunogen:
A 16 residue synthetic MAP peptide KKDDKGQQLPFEVQVG based on the C. trachomatis cpn10 (residues 54-69) (serovar A/HAR-13)
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.1mM PMSF, before the addition of glycerol to 40%.
Specificity:
Detects a 15kD protein, corresponding to the apparent molecular mass of cpn10 on SDS-PAGE immunoblots, in C. trachomatis infected cells. This antibody will not react with the human or mouse cpn10 homolog nor with E. coli GroES.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. LaVerda, D. and Byrne, G.I. (1997) J. Clin. Micro. 35: 1209-1215.
2. Morrison, R.P., Belland, R.J., Lyng, K., and Caldwell, H.D. (1989) J. Exp. Med. 170:
1271-1283.