Technical Data
C3470B
Chaperonin 10 (Cpn10, Heat Shock Protein 10, HSP10) (Chlamydia)
Description:
Homologs of E. coli GroES make up a family of small heat shock proteins with an approximate molecular mass of 10kD (Hsp10s). Hsp10 acts as a cochaperone and interacts with members of the Hsp60 family (GroEL in E. coli) to promote proper folding of polypeptides. In chlamydia, cpn10 is thought to interact with cHsp60, a reported immunopathological antigen (2), identifying cpn10 as a potential contributor to an immunological response (1).

Applications:
Western Blot (Colorimetric) (1): 0.25ug/ml
Immunoprecipitation (1)
ELISA (1)
Optimal dilutions to be determined by researcher.
TypeIsotypeCloneGrade
MabIgG14E45Affinity Purified
SizeStorageShippingSourceHost
25ug-20°CBlue IceMouse
Concentration:
Immunogen:
A 16 residue synthetic MAP peptide KKDDKGQQLPFEVQVG based on the C. trachomatis cpn10 (residues 54-69) (serovar A/HAR-13)
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.1mM PMSF.
Specificity:
This affinity purified antibody detects a 15kD protein, corresponding to the apparent molecular mass of cpn10 on SDS-PAGE immunoblots, in C. trachomatis infected cells. This antibody will not react with the human or mouse cpn10 homolog nor with E. coli GroES.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. LaVerda, D. and Byrne, G.I. (1997) J. Clin. Micro. 35: 1209-1215.
2. Morrison, R.P., Belland, R.J., Lyng, K., and Caldwell, H.D. (1989) J. Exp. Med. 170:
1271-1283.