Homologs of E. coli GroES make up a family of small heat shock proteins with an approximate molecular mass of 10kD (Hsp10s). Hsp10 acts as a cochaperone and interacts with members of the Hsp60 family (GroEL in E. coli) to promote proper folding of polypeptides. In chlamydia, cpn10 is thought to interact with cHsp60, a reported immunopathological antigen (2), identifying cpn10 as a potential contributor to an immunological response (1).
Western Blot (Colorimetric) (1): 0.25ug/ml
Optimal dilutions to be determined by researcher.
|A 16 residue synthetic MAP peptide KKDDKGQQLPFEVQVG based on the C. trachomatis cpn10 (residues 54-69) (serovar A/HAR-13) |
|Purified by Protein G affinity chromatography.|
|Supplied as a liquid in PBS, 0.1mM PMSF. |
|This affinity purified antibody detects a 15kD protein, corresponding to the apparent molecular mass of cpn10 on SDS-PAGE immunoblots, in C. trachomatis infected cells. This antibody will not react with the human or mouse cpn10 homolog nor with E. coli GroES. |
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. LaVerda, D. and Byrne, G.I. (1997) J. Clin. Micro. 35: 1209-1215. |
2. Morrison, R.P., Belland, R.J., Lyng, K., and Caldwell, H.D. (1989) J. Exp. Med. 170: