Technical Data
C3967-01C
CHFR (Checkpoint with Forkhead and Ring Finger Domains, E3 Ubiquitin Protein Ligase CHFR, FLJ10796, FLJ33629, RNF116, RNF196) (Azide Free)
Description:
Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (paraffin): 1.5ug/ml
ELISA: 1ug/ml-3ng/ml
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG110B2217Affinity Purified
SizeStorageShippingSourceHost
100ul4C/-20C (No Glycerol)Blue IceHumanMouse
Concentration:
As reported
Immunogen:
Partial recombinant protein corresponding to a portion of amino acids within aa1-653 of human CHFR with a GST tag.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added (Azide free).
Specificity:
Recognizes human CHFR.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Privette, L. M. et al (2008) Loss of CHFR in human mammary epithelial cells causes genomic instability by disrupting the mitotic spindle assembly checkpoint. Neoplasia. 10: 643-52.