Technical Data
Media
Chick Fibroblast Basal Medium MCDB 202 w/o HEPES (Powder)
C4000-01
Cell Culture Grade
Storage RT    Shipping RT
Components shown as mg/liter
Inorganic Salts:
Ammonium Metavandate0.000585
Ammonium Molybdate0.0012359
Calcium Chloride•2H2022.00
Cupric Sulfate •5H200.0002497
Ferric Nitrate•9H201.39
Magnesium Sulfate180.57
Manganese Sulfate•5H2O0.0001205
Nickel Chloride Hexahydrate0.0000011885
Potassium Chloride186.25
Potassium Phosphate Mono68.05
Sodium Chloride7183.2
Sodium Metasilicate•9H2O0.1421
Sodium Phosphate Dibasic71.05
Sodium Selenite0.00789
Stannous Chloride Dihydrate0.000001128
Zinc Sulfate•7H2O0.02875
Amino Acids:
L-Alanine8.90
L-Arginine52.26
L-Asparagine132.10
L-Aspartic Acid13.31
L-Cysteine24.44
L-Glutamic Acid14.71
L-Glutamine146.00
Glycine7.51
L-Histidine15.52
L-Isoleucine13.12
L-Leucine39.36
L-Lysine29.24
L-Methionine4.48
L-Phenylalanine4.96
L-Proline5.76
L-Serine31.53
L-Threonine35.73
L-Tryptophan6.13
L-Tyrosine9.06
L-Valine35.16
Vitamins:
Biotin0.00733
Choline Chloride13.96
Vitamin B120.1355
Folinic Acid, Calcium0.0006016
Myo-Inositol18.02
Nicotinamide6.10
D-Pantothenic Acid•Ca0.2383
Pyridoxine•HCl0.0616
Riboflavin0.1129
Thiamine•HCl0.337
Other:
Adenine0.135
Thymidine0.07266
D-Glucose1440.0
HEPES Free AcidAbsent
Linoleic Acid0.0561
Lipoic Acid0.00206
Phenol Red, Sodium1.242
Putrescine•2HCl0.0001611
Sodium Pyruvate55.00
Total:9.877g/liter
Basal Medium MCDB 202 is specifically formulated for the cultivation of chick fibroblasts in accordance with the formulation by McKeehan and Ham. Supplement with growth factors or fetal calf serum (0.2-2.0% dialyzed).


Directions per Liter: Dissolve 9.877g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 1.2g sodium bicarbonate while stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Light tan, homogenous, free flowing powder.

Solubility (9.877g/L): Yellow, clear, complete

pH: As Reported

Endotoxin: ~10EU/mg


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Mckeehan, W.L., Ham, R.G., Dev. Biol. Std. 37: 97-98 (1977).