		Technical Data
C4125-03E	CHIP, NT (E3 Ubiquitin-protein Ligase CHIP, Carboxy Terminus of HSP70-interacting Protein, CLL-associated Antigen KW-8, STIP1 Homology and U-Box Containing Protein 1, Antigen NY-CO-7, STUB1, PP1131)

Description:
CHIP is an E3 ligase for nNOS whose action is facilitated by (and possibly requires) its interaction with nNOS-bound hsp70. Co-chaperone CHIP, possibly with another E3 ligase(s), modulates the ubiquitylation of mutant Cu/Zn-superoxide dismutase and renders them more susceptible for proteasomal degradation. CHIP functions as a negative regulator of AR transcriptional activity by promoting AR degradation. CHIP-Hsc70 complex ubiquitinates phosphorylated tau and enhances cell survival. CHIP can interact with the Smad1/Smad4 proteins and block BMP signal transduction through the ubiquitin-mediated degradation of Smad proteins. CHIP E3 controls both the association of Hsp70/Hsp90 chaperones with ErbB2 and the down-regulation of ErbB2 induced by inhibitors of Hsp90. CHIP is associated with Parkin and enhances its ubiquitin ligase activity related to Parkinson's disease.

Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

Synthetic peptide at the N-term of human CHIP (STUB1) (KLH).

Purity:

Purified by ammonium sulfate precipitation.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human CHIP.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1.Peng,H.M., et al. J. Biol. Chem. 279(51), 52970-52977 (2004) 2.Alberti,S., et al. Mol. Biol. Cell 15(9), 4003-4010 (2004) 3.Beausoleil,S.A., et al. PNAS 101(33), 12130-12135 (2004) 4.He,B., et al. J. Biol. Chem. 279(29), 30643-30653 (2004) 5.Petrucelli,L., et al. Hum. Mol. Genet. 13(7), 703-714 (2004) 6.Shimura,H., et al. J. Biol. Chem. 279(6), 4869-4876 (2004) 7.Li,L., et al. Mol. Cell. Biol. 24(2), 856-864 (2004) 8.Zhou,P., et al. J. Biol. Chem. 278(16), 13829-13837 (2003) 9.Imai,Y., et al. Mol. Cell 10(1), 55-67 (2002) 10.Ballinger,C.A., et al. Mol. Cell. Biol. 19(6), 4535-4545 (1999).