Technical Data
C4125-03F
CHIP, CT (E3 Ubiquitin-protein Ligase CHIP, Carboxy Terminus of HSP70-interacting Protein, CLL-associated Antigen KW-8, STIP1 Homology and U-Box Containing Protein 1, Antigen NY-CO-7, STUB1, PP1131)
Description:
CHIP is an E3 ligase for nNOS whose action is facilitated by (and possibly requires) its interaction with nNOS-bound hsp70. Co-chaperone CHIP, possibly with another E3 ligase(s), modulates the ubiquitylation of mutant Cu/Zn-superoxide dismutase and renders them more susceptible for proteasomal degradation. CHIP functions as a negative regulator of AR transcriptional activity by promoting AR degradation. CHIP-Hsc70 complex ubiquitinates phosphorylated tau and enhances cell survival. CHIP can interact with the Smad1/Smad4 proteins and block BMP signal transduction through the ubiquitin-mediated degradation of Smad proteins. CHIP E3 controls both the association of Hsp70/Hsp90 chaperones with ErbB2 and the down-regulation of ErbB2 induced by inhibitors of Hsp90. CHIP is associated with Parkin and enhances its ubiquitin ligase activity related to Parkinson's disease.

Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide at the C-term of human CHIP (STUB1) (KLH).
Purity:
Purified by ammonium sulfate precipitation.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human CHIP.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Peng,H.M., et al. J. Biol. Chem. 279(51), 52970-52977 (2004) 2.Alberti,S., et al. Mol. Biol. Cell 15(9), 4003-4010 (2004) 3.Beausoleil,S.A., et al. PNAS 101(33), 12130-12135 (2004) 4.He,B., et al. J. Biol. Chem. 279(29), 30643-30653 (2004) 5.Petrucelli,L., et al. Hum. Mol. Genet. 13(7), 703-714 (2004) 6.Shimura,H., et al. J. Biol. Chem. 279(6), 4869-4876 (2004) 7.Li,L., et al. Mol. Cell. Biol. 24(2), 856-864 (2004) 8.Zhou,P., et al. J. Biol. Chem. 278(16), 13829-13837 (2003) 9.Imai,Y., et al. Mol. Cell 10(1), 55-67 (2002) 10.Ballinger,C.A., et al. Mol. Cell. Biol. 19(6), 4535-4545 (1999).