Technical Data
Chitotriosidase (Chitinase 1, CHIT-1)
Human CHIT-1 (chitotriosidase-1; also chitinase-1) is a 50kD, secreted glycoprotein that belongs to glycosylhydrolase family #18. It is synthesized by macrophages, and may serve to degrade chitin (poly-N-acetylglucosamine)-containing pathogens such as fungi and nematodes. Mature CHIT-1 is 435 amino acids (aa) in length, and contains a catalytic domain (aa22-372) followed by a chitin-binding region (aa373-466). Very high levels of CHIT1 are found in the plasma of patients with Gaucher's disease type I. Mature human CHIT-1 is 79% aa identical to mouse CHIT-1.

Suitable for use in Direct ELISA, Immunoprecipitation and Neutralization. Other applications not tested.

Recommended Dilutions:
ELISA (Direct): 0.5-1.0ug/ml. Detection limit for rhCHIT1 is approximately 2ng/well.
Immunoprecipitation: 25ug/ml to immunoprecipitate rhCHIT1 from conditioned cell culture medium.
Neutralization: The antibody was preincubated with the enzyme at different molar ratios at RT for 30 min. The enzyme was then assayed with 4-MU-beta-D-N,N',N''-triacetylchitotriose. 50% of the enzymatic activity was inhibited by the antibody at approximately 56ng/ml (IC 50) under conditions in which the enzyme was present at 20ng/ml and the substrate concentration was 0.01mM. IC 50 was achieved at approximately 1:1 molar ratio of the antibody to the enzyme.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG17H206Affinity Purified
100ug-20CBlue IceHumanMouse
~0.5mg/ml (after reconstitution)
Recombinant protein corresponding to aa22-466 of human CHIT1, expressed in NS0 cells (NP_003456). Species Sequence Homology: mouse; 79%.
Purified by Protein G affinity chromatography.
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Recognizes human CHIT1 in direct ELISAs and Western Blots. In these formats, this antibody shows no crossreactivity with recombinant human CHI-3L1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.