Technical Data
C4164-50
Chitotriosidase (Chitinase 1, CHIT-1)
Description:
Human CHIT-1 (chitotriosidase-1; also chitinase-1) is a 50kD, secreted glycoprotein that belongs to glycosylhydrolase family #18. It is synthesized by macrophages, and may serve to degrade chitin (poly-N-acetylglucosamine)-containing pathogens such as fungi and nematodes. Mature CHIT-1 is 435 amino acids (aa) in length, and contains a catalytic domain (aa #22-372) followed by a chitin-binding region (aa #373-466). Very high levels of CHIT1 are found in the plasma of patients with Gaucher's disease type I. Mature human CHIT-1 is 79% aa identical to mouse CHIT-1.
Applications:
Suitable for use in ELISA, Immunoprecipitation and Neutralization. Other applications not tested.

Recommended Dilution:
ELISA (direct): 0.5-1.0ug/ml. Detection limit for rhCHIT1 is approximately 2ng/well.
Immunoprecipitation: 25ug/ml to immunoprecipitate rhCHIT1 from conditioned cell culture medium.
Neutralization: The antibody was preincubated with the enzyme at different molar ratios at RT for 30 min. The enzyme was then assayed with 4-MU-beta-D-N,N',N''-triacetylchitotriose. 50% of the enzymatic activity was inhibited by the antibody at approximately 56ng/mL (IC 50) under conditions in which the enzyme was present at 20ng/mL and the substrate concentration was 0.01mM. IC 50 was achieved at approximately 1:1 molar ratio of the antibody to the enzyme.
Optimal dilutions to be determined by the researcher.

Hybridoma:
Fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human CHIT1.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute by adding sterile 40-50% glycerol, PBS. Aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG17H206Affinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
NS0-derived rhCHIT-1
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile 40-50% glycerol, PBS.
Specificity:
Recognizes human CHIT1 in direct ELISAs and Western Blots. In these formats, this antibody shows no crossreactivity with rhCHI-3L1. Species sequence homology: mouse: 79%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.