Technical Data
C4250-11
Chlamydia trachomatis (HRP)
Description:
Chlamydia is caused by the bacterium Chlamydia trachomatis. The intracytoplasmic inclusions caused by the bacterium are draped around the infected cells nucleus. Chlamydia has a genome size of approximately 500-1000 kilobases and contains both RNA and DNA. The organism is also extremely temperature sensitive and must be refrigerated at 4C as soon as a sample is obtained. Colonization of Chlamydia begins with attachment to sialic acid receptors on the eye, throat or genitalia. It persists at body sites that are inaccessible to phagocytes, T-cells, and B-cells. It also exists as 15 different serotypes. These serotypes cause four major diseases in humans: endemic trachoma (caused by serotypes A and C), sexually transmitted disease and inclusion conjunctivitis (caused by serotypes D and K), and lymphogranuloma venereum (caused by serotypes L1, L2, and L3). Studies reveal that Chlamydia, because of its cell wall, is able to inhibit phagolysosome fusion in phagocytes. The cell wall is proposed to be gram-negative in that it contains an outer lipopolysaccharide membrane, but it lacks peptidoglycan in its cell wall. This lack of peptidoglycan is shown by the inability to detect muramic acid and antibodies directed against it. It may, however, contain a carboxylated sugar other than muramic acid. The proposed structure consists of a major outer membrane protein cross-linked with disulfide bonds. It also contain cysteine-rich proteins (CRP) that may be the functional equivalent to peptidoglycan. This unique structure allows for intracellular division and extracellular survival (Hatch 1996).
-Kenneth Todar, University of Wisconsin-Madison

Applications: Suitable for Immunohistochemistry, Western Blot and ELISA

Recommended Dilution:
Immunohistochemistry: 1:20-1:200
Western Blot: 1:20-1:200
Indirect Immunofluorescence: 1:1000-3000 (vs. all serovars A-K, L1-L3)

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.


TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
1ml-20CBlue IceRabbit
Concentration:
~1-3 mg/ml
Immunogen:
L2 + other serovar groups
Purity:
Purified IgG fraction covalently coupled to a highly purified preparation of horseradish peroxidase (RZ>3). Free enzyme is absent
Form
Supplied as a liquid in PBS, pH 7.2, PBS, 10 mg/ml BSA, 0.002% Thimerosal.
Specificity:
Purified elementary bodies, disrupted. Crossreacts with Chlamydia psittacii & Chlamydia pneumoniae (TWAR). Negative vs. Hep-2 cells and egg yolk sac.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.