Technical Data
C4250-51D
Chlamydia LPS
Description:
Chlamydia is caused by the bacterium Chlamydia trachomatis. The intracytoplasmic inclusions caused by the bacterium are draped around the infected cells nucleus. Chlamydia has a genome size of approximately 500-1000kB and contains both RNA and DNA. The organism is also extremely temperature sensitive and must be refrigerated at 4C as soon as a sample is obtained. Colonization of Chlamydia begins with attachment to sialic acid receptors on the eye, throat or genitalia. It persists at body sites that are inaccessible to phagocytes, T-cells, and B-cells. It also exists as 15 different serotypes. These serotypes cause four major diseases in humans: endemic trachoma (caused by serotypes A and C), sexually transmitted disease and inclusion conjunctivitis (caused by serotypes D and K), and lymphogranuloma venereum (caused by serotypes L1, L2, and L3). Studies reveal that Chlamydia, because of its cell wall, is able to inhibit phagolysosome fusion in phagocytes. The cell wall is proposed to be gram-negative in that it contains an outer lipopolysaccharide membrane, but it lacks peptidoglycan in its cell wall. This lack of peptidoglycan is shown by the inability to detect muramic acid and antibodies directed against it. It may, however, contain a carboxylated sugar other than muramic acid. The proposed structure consists of a major outer membrane protein cross-linked with disulfide bonds. It also contain cysteine-rich proteins (CRP) that may be the functional equivalent to peptidoglycan. This unique structure allows for intracellular division and extracellular survival (Hatch 1996).
-Kenneth Todar, University of Wisconsin-Madison

Applications:
Direct ELISA

Recommended Dilution:
Direct ELISA: 1:10,000 dilution will yield 0.2 O.D. using alkaline phosphatase conjugated rabbit anti-mouse Ig (against purified Chalmidia LPS)

Reconstitution:
Reconstitute with 0.5ml of dH2O. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b5C34Highly Purified
SizeStorageShippingSourceHost
500ug-20CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
Purified Chlamidia LPS
Purity:
Purified by ion exchange chromatography.
Form
Supplied as a lyophilized powder in PBS, pH 7.2.
Specificity:
Recognizes Chlamydia LPS.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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