Technical Data
C4250-52K
Chlamydia trachomatis L2 (HRP)
Description:
Chlamydia is caused by the bacterium Chlamydia trachomatis. The intracytoplasmic inclusions caused by the bacterium are draped around the infected cells nucleus. Chlamydia has a genome size of approximately 500-1000kb and contains both RNA and DNA. The organism is also extremely temperature sensitive and must be refrigerated at 4C as soon as a sample is obtained. Colonization of Chlamydia begins with attachment to sialic acid receptors on the eye, throat or genitalia. It persists at body sites that are inaccessible to phagocytes, T-cells and B-cells. It exists as 15 different serotypes that cause four major diseases in humans: endemic trachoma (serotypes A and C), sexually transmitted disease and inclusion conjunctivitis (serotypes D and K), and lymphogranuloma venereum (serotypes L1, L2, and L3). Chlamydia, because of its cell wall, is able to inhibit phagolysosome fusion in phagocytes. The cell wall is proposed to be gram-negative in that it contains an outer lipopolysaccharide membrane, but it lacks peptidoglycan in its cell wall. This lack of peptidoglycan is evident in its inability to detect muramic acid and antibodies directed against it. The proposed structure consists of a major outer membrane protein cross-linked with disulfide bonds. It also contains cysteine-rich proteins (CRP) that may be the functional equivalent to peptidoglycan. This unique structure allows for intracellular division and extracellular survival.

Applications:
Suitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:200-1:1000
Western Blot: 1:20-1:200
Immunocytochemistry: 1:20-1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
1ml4C (-20C Glycerol)Blue IceRabbit
Concentration:
~1-2mg/ml
Immunogen:
Chlamydia trachomatis L2 + other serovar groups
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.002% thimerosal. Labeled with horseradish peroxidase (HRP).
Specificity:
Recognizes purified elementary bodies, disrupted. Species Crossreactivity: Chlamydia psittacii and Chlamydia pneumoniae (TWAR). Uninfected Cell Reactivity: Negative vs. HEp-2 cells and egg yolk sac.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.