Technical Data
C4250-53
Chlamydia trachomatis
Description:
Chlamydia is caused by the bacterium Chlamydia trachomatis. The intracytoplasmic inclusions caused by the bacterium are draped around the infected cells nucleus. Chlamydia has a genome size of approximately 500-1000 kilobases and contains both RNA and DNA. Colonization of Chlamydia begins with attachment to sialic acid receptors on the eye, throat or genitalia. It persists at body sites that are inaccessible to phagocytes, T-cells, and B-cells. It also exists as 15 different serotypes. These serotypes cause four major diseases in humans: endemic trachoma (caused by serotypes A and C), sexually transmitted disease and inclusion conjunctivitis (caused by serotypes D and K), and lymphogranuloma venereum (caused by serotypes L1, L2, and L3). Studies reveal that Chlamydia, because of its cell wall, is able to inhibit phagolysosome fusion in phagocytes. The cell wall is proposed to be gram-negative in that it contains an outer lipopolysaccharide membrane, but it lacks peptidoglycan in its cell wall. This lack of peptidoglycan is shown by the inability to detect muramic acid and antibodies directed against it. It may, however, contain a carboxylated sugar other than muramic acid. The proposed structure consists of a major outer membrane protein cross-linked with disulfide bonds. It also contain cysteine-rich proteins (CRP) that may be the functional equivalent to peptidoglycan. This unique structure allows for intracellular division and extracellular survival.
-Kenneth Todar, University of Wisconsin-Madison

Applications:
Suitable for use in Immunoflorescence and ELISA. Other applications not tested.

Recommended Dilution:
IFA: 1:1000
Optimal dilutions to be determined by the researcher.

Matched Pairs:
Capture: C4250-56
Detection: C4250-53

Recommended Antibody:
Capture: C4250-56: Chlamydia trachomatis LPS (Glycerol Free)

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
1mg-20CBlue IceGoat
Concentration:
~2mg/ml
Immunogen:
Chlamydia trachomatis, Elementary Bodies.
Purity:
Purified IgG fraction
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity:
Recognizes elementary bodies of all strains of Chlamydia trachomatis. Crossreacts with C. pneumoniae and C. psittacii.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.