Technical Data
C4275-35C
Cholera Toxin, beta (HRP)
Description:
The Beta subunit of cholera toxin binds to a GM1-ganglioside receptor which is widely accepted to initiate toxin action by triggering uptake and delivery of the toxin Alpha subunit into cells. The holotoxin consists of a pentameric ring of Beta subunits whose central pore is occupied by the Alpha subunit. The Alpha subunit contains two chains, A1 and A2, linked by a disulfide bridge. The Alpha subunit (and Cholera toxin) activate the adenylate cyclase enzyme in cells of the intestinal mucosa leading to increased levels of intracellular cAMP.

Applications:
Suitable for use in ELISA and Western Blot. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1:20-1:200
ELISA: 1:200-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
500ul-20CBlue IceRabbit
Concentration:
~1mg/ml
Immunogen:
Cholera Toxin, beta epitope (purified choleragenoid).
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.002% Thimerosal. Labeled with HRP.
Specificity:
Recognizes the beta subunit of Cholera toxin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.