Technical Data
C4275-35H
Cholera Toxin, alpha, Vibrio cholerae
Description:
The cholera holotoxin consists of a pentameric ring of Beta subunits whose central pore is occupied by the Alpha subunit. The Alpha subunit contains two chains, A1 and A2, linked by a disulfide bridge. The Alpha subunit (and Cholera toxin) activates the adenylate cyclase enzyme in cells of the intestinal mucosa leading to increased levels of intracellular cAMP.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1905-18D IgM (X-Adsorbed) (FITC) Pab GtxMo
I1905-28A IgM (X-Adsorbed) (HRP) Pab GtxMo

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgM3j3Purified
SizeStorageShippingSourceHost
200ug-20°CBlue IceMouse
Concentration:
Immunogen:
Vibrio cholerae extract
Purity:
Purified by ammonium sulfate precipitation.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity:
Recognizes the alpha chain of the V. cholerae toxin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.