Technical Data
C4275-35P
Cholera Toxin, beta (FITC)
Description:
Cholera toxin is comprised of two subunits, A and B. Subunit A is an ADP-ribosyltransferase, which disrupts the proper signaling of G proteins, leading to cell dehydration. The 12kD nontoxic subunit B ("choleragenoid"), which is assembled into a 60kD pentamer above pH 2, allows the protein complex to bind to cellular surfaces via the pentasaccharide chain of ganglioside GM1.

Applications:
Suitable for use in ELISA, Immunofluorescence, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: >1:1,000
Immunofluorescence: 1:10-1:50. Direct FA staining of target antigens in a permissive tissue culture system. Acetone fixation of the antigen source is recommended prior to staining.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGHighly Purified
SizeStorageShippingSourceHost
1ml-20°CBlue IceRabbit
Concentration:
~4-5mg/ml
Immunogen:
Purified Choleragenoid
Purity:
Purified IgG fraction to a purity of > 95%.
Form
Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.1% sodium azide. Labeled with FITC.
Specificity:
Recognizes Beta subunit of Cholera toxin. Immunoprecipitates toxin in gel techniques.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Edwards, K.A and March, J.C. (2007) Analytical Biochemistry 368:39-48.