Technical Data
Cholera Toxin, beta (Cholera Enterotoxin beta, Choleragenoid, CTX B, CTXB, CTX-B, TOXB, VC1456) (FITC)
Cholera toxin is comprised of two subunits, A and B. Subunit A is an ADP-ribosyltransferase, which disrupts the proper signaling of G proteins, leading to cell dehydration. The 12kD nontoxic subunit B ("choleragenoid"), which is assembled into a 60kD pentamer above pH 2, allows the protein complex to bind to cellular surfaces via the pentasaccharide chain of ganglioside GM1.

Suitable for use in FLISA, Immunofluorescence, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
FLISA: >1:1,000
Immunofluorescence: 1:10-1:50. Direct FA staining of target antigens in a permissive tissue culture system. Acetone fixation of the antigen source is recommended prior to staining.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGHighly Purified
1ml-20CBlue IceRabbit
Purified Choleragenoid
Purified IgG fraction to a purity of > 95%.
Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.09% sodium azide. Labeled with FITC.
Recognizes Beta subunit of Cholera toxin. Immunoprecipitates toxin in gel techniques.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Edwards, K.A and March, J.C. (2007) Analytical Biochemistry 368:39-48.