Technical Data
C4275-35Q
Cholera Toxin, beta (HRP)
Description:
Cholera toxin is comprised of two subunits, A and B. Subunit A is an ADP-ribosyltransferase, which disrupts the proper signaling of G proteins, leading to cell dehydration. The 12kD nontoxic subunit B ("choleragenoid"), which is assembled into a 60kD pentamer above pH 2, allows the protein complex to bind to cellular surfaces via the pentasaccharide chain of ganglioside GM1.

Applications:
Suitable for use in ELISA, Western Blot, Immunocytochemistry, and Immunoprecipitation (Gel). Other applications not tested.

Recommended Dilution:
Immunocytochemistry: 1:20-1:200
Western Blot: 1:20-1:200
ELISA: 1:200-1:000
Optimal dilutions to be determined by the researcher.

End-Point Titer: >1:1000 by ELISA

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGHighly Purified
SizeStorageShippingSourceHost
1ml-20°CBlue IceRabbit
Concentration:
~1-2mg/ml
Immunogen:
Purified Choleragenoid.
Purity:
Purified IgG fraction to a purity of ~95%.
Form
Supplied as a liquid in PBS, 10mg/ml BSA, 0.002% thimerosal. Labeled with HRP. Estimated molar HRP:IgG ratio: 2-3.
Specificity:
Recognizes the beta subunit of Cholera toxin. Immunoprecipitates toxin in gel techniques.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.