Technical Data
Cholera Toxin, beta (Cholera Enterotoxin beta, Choleragenoid, CTX B, CTXB, CTX-B, TOXB, VC1456) (HRP)
Cholera toxin is comprised of two subunits, A and B. Subunit A is an ADP-ribosyltransferase, which disrupts the proper signaling of G proteins, leading to cell dehydration. The 12kD nontoxic subunit B ("choleragenoid"), which is assembled into a 60kD pentamer above pH 2, allows the protein complex to bind to cellular surfaces via the pentasaccharide chain of ganglioside GM1.

Suitable for use in ELISA, Western Blot, Immunocytochemistry, and Immunoprecipitation (Gel). Other applications not tested.

Recommended Dilution:
Immunocytochemistry: 1:20-1:200
Western Blot: 1:20-1:200
ELISA: 1:200-1:000
Optimal dilutions to be determined by the researcher.

End-Point Titer: >1:1000 by ELISA

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGHighly Purified
1ml-20CBlue IceRabbit
Purified Choleragenoid.
Purified IgG fraction to a purity of ~95%.
Supplied as a liquid in PBS, 10mg/ml BSA, 0.002% thimerosal. Labeled with HRP. Estimated molar HRP:IgG ratio: 2-3.
Recognizes the beta subunit of Cholera toxin. Immunoprecipitates toxin in gel techniques.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.