		Technical Data
C4275-35U	Cholera Toxin, beta (Beta Subunit Cholera Toxin, Cholera Enterotoxin beta Chain, Cholera Enterotoxin B Chain, Cholera Enterotoxin gamma Chain, Cholera Enterotoxin Subunit B, Cholera Toxin B Protein, Choleragenoid, CTX B, CTXB, CTX-B, TOXB, VC1456)

Description:
The B subunit of cholera toxin (CtxB) binds to a GM1-ganglioside receptor, a ubiquitous glycolipid cell surface receptor. This binding is widely accepted to initiate toxin action by triggering uptake and delivery of the toxin A subunit into cells. The beta chain has no toxic activity by itself. The holotoxin consists of a pentameric ring of B subunits whose central pore is occupied by the A subunit. The A subunit contains two chains, A1 and A2, linked by a disulfide bridge.

The A subunit (and Cholera toxin) activates the adenylate cyclase enzyme in cells of the intestinal mucosa leading to increased levels of intracellular cAMP.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	11H70	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice		Mouse

Concentration:

As reported

Immunogen:

Purified Cholera toxin B subunit

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

Specificity:

Recognizes the B subunit of cholera toxin and reacts with the intact toxin.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.