Technical Data
Cholera Toxin, beta (Cholera Enterotoxin beta, Choleragenoid, CTX B, CTXB, CTX-B, TOXB, VC1456)
The Beta subunit of cholera toxin binds to a GM1-ganglioside receptor which is widely accepted to initiate toxin action by triggering uptake and delivery of the toxin Alpha subunit into cells. The holotoxin consists of a pentameric ring of Beta subunits whose central pore is occupied by the Alpha subunit. The Alpha subunit contains two chains, A1 and A2, linked by a disulfide bridge. The Alpha subunit (and Cholera toxin) activate the adenylate cyclase enzyme in cells of the intestinal mucosa leading to increased levels of intracellular cAMP.

Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:20-1:200
Optimal dilutions to be determined by the researcher.

Matched Pairs for ELISA:
C4275-35Y and C4275-35S
C4275-35Y and C4275-39H

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG112K223Affinity Purified
100ug-20CBlue IceMouse
Purified native B subunit.
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Recognizes the B subunit of cholera toxin. Reacts with intact toxin. Does not cross-react with E. coli heat labile toxin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.