Technical Data
C4275-35Y
Cholera Toxin, beta (Beta Subunit Cholera Toxin, Cholera Enterotoxin beta Chain, Cholera Enterotoxin B Chain, Cholera Enterotoxin gamma Chain, Cholera Enterotoxin Subunit B, Cholera Toxin B Protein, Choleragenoid, CTX B, CTXB, CTX-B, TOXB, VC1456)
Description:
The Beta subunit of cholera toxin binds to a GM1-ganglioside receptor which is widely accepted to initiate toxin action by triggering uptake and delivery of the toxin Alpha subunit into cells. The holotoxin consists of a pentameric ring of Beta subunits whose central pore is occupied by the Alpha subunit. The Alpha subunit contains two chains, A1 and A2, linked by a disulfide bridge. The Alpha subunit (and Cholera toxin) activate the adenylate cyclase enzyme in cells of the intestinal mucosa leading to increased levels of intracellular cAMP.

Applications:
Suitable for use in Immunofluorescence, Immunohistochemistry, ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:10-1:50
ELISA: 1:20-1:200
Western Blot: 1:10-1:50
Immunohistochemistry: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG112K223Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouse
Concentration:
~0.1mg/ml
Immunogen:
Purified native B subunit.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity:
Recognizes the B subunit of cholera toxin. Reacts with intact toxin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.