Technical Data
C4275-39J
Cholera Toxin
Description:
Cholera toxin is comprised of two subunits, A and B. Subunit A is an ADP-ribosyltransferase, which disrupts the proper signaling of G proteins, leading to cell dehydration. The 12kD nontoxic subunit B ("choleragenoid"), which is assembled into a 60kD pentamer above pH 2, allows the protein complex to bind to cellular surfaces via the pentasaccharide chain of ganglioside GM1.


Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:20-1:200
Optimal dilutions to be determined by the researcher.

Matched Pairs (capture/detection):
C4275-39H / C4275-39H
C4275-39J / C4275-39H
C4275-39J / C4275-39K

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG18D122Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouse
Concentration:
~0.1mg/ml
Immunogen:
Cholera toxin
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 0.01M PBS, pH 7.2, 0.1% sodium azide.
Specificity:
Recognizes the B subunit of cholera toxin. Reacts with intact toxin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.