Technical Data
C4350-02
Chloramphenicol
Description:
Chloramphenicol is a chlorine-containing antibiotic produced by Streptomyces venezuelae. It has a wide spectrum of activity against gram-positive and gram-negative cocci and bacilli (including anaerobes), Rickettsia, Mycoplasma, and Chlamydia. It inhibits prokaryotic protein synthesis by attaching to the 50S ribosomal subunit. This inhibits peptidyltransferase, thereby preventing the formation of peptide bonds. It also inhibits protein synthesis in mitochondria which accounts for its toxic effects that cause aplastic anemia. Chloramphenicol has other adverse effects such as bone marrow depression, and gray baby syndrome. Its use is limited to serious infections such as those where resistance to other antibiotics occurs. It is still widely used in the treatment of typhoid fever, meningitis and eye infections.

Applications:
Suitable for use in ELISA and Fluoroimmunoassay (FIA). Other applications have not been tested.

Recommended Dilution:
Indirect ELISA: 1:2000; Coat with 400ng of chloramphenicol-BSA per well.
FIA (Polarization Fluoroimmunoassay): 1:750; Tracer is aromatic amino derivative of chloramphenicol labeled by reaction with fluorescein isothiocyanate (10nmol/L).
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
1ml4C (-20C Glycerol)Blue IceSheep
Concentration:
~10-12mg/ml
Immunogen:
Aromatic amino derivative of chloramphenicol (prepared by reduction of the nitro group by high-pressure hydrogenation) coupled to KLH, using the diazotization procedure.
Purity:
Purified by ammonium sulfate frationation.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, before the addition of glycerol to 40%.
Specificity:
Recognizes Chloramphenicol.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.