Technical Data
C5045-35
Cholesterol Acyltransferase 1 (Acyl Coenzyme A Cholesterol Acyltransferase 1, ACAT1, Sterol O-Acyltransferase 1)
Description:
ACAT1 is a ~50kD protein that catalyzes the formation of intracellular cholesterol esters in various tissues, such as adult human liver, adrenal glands, macrophages, and kidneys.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500.
Immunohistochemistry: 1:1000.
Immunocytochemistry: 1:1000.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
1Vial4C (-20C Glycerol)Dry IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa 6-23 (KMSLRNRLSKSRENPEED) of human ACAT-1 (Acyl-CoA: cholesterol acyltransferase-1)
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in TBS, pH 7.4, 0.5mg/ml BSA and 0.02% sodium azide, 50% glycerol.
Specificity:
Recognizes human, mouse, rat and pig ACAT-1. Other species have not been tested.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.