Technical Data
C5057
Choline Acetyltransferase
Description:
Acetylcholine (Ach) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransfrase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-cholinacetyltransferase a useful specific marker.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: 10-20ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG12Q38Purified
SizeStorageShippingSourceHost
50ug-20CBlue IcePorcineMouse
Concentration:
~1mg/ml
Immunogen:
Highly purified ChAT obtained from porcine brain
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.
Specificity:
Recognizes porcine choline acetyltransferase (ChAT)-positive cells. Species Crossreactivity: human and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ostermann, et al., Eur. J. Biochem. 192: 215 (1990). 2. Ostermann-Fatif, et al., Journal of Biochemistry 58: 1060 (1992). 3. Watson, et al., Peptides 7: 155 (1986). 4. Eckenstein, F. & Thoenen, H., EMBO J. 1: 363 (1982). 5. Houser, C.R., et al., Brain Res. 266: 97 (1983). 6. Ostermann-Fatif, et al., Journal of Immunological Methods 157: 73 (1992).