Technical Data
C5057-02E
Choline Acetyltransferase (ChAT, Choline O-acetyltransferase, Acetyl CoA Choline O-acetyltransferase, CHOACTase, Choline Acetylase, CMS1A, CMS1A2, EC 2.3.1.6)
Description:
Acetylcholine (ACh) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetylase, aka choline acetyltransferase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-cholinacetyltransferase a useful specific marker

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml
Immunohistochemistry: 1:200
Optimal dilutions to be determined by the researcher.

Positive Control:
Human fetal brain tissue lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug4C/-20CBlue IceHumanRabbit
Concentration:
~0.1mg/ml
Immunogen:
Recombinant protein corresponding to the C-terminus of human Choline acetylase (GST-tagged).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes human Choline acetylase. Species Crossreactivity: rat, mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.