Technical Data
C5057-03
Choline Acetyltransferase (ChAT)
Description:
Murine choline-O-acetyltransferase (EC 2.3.1.6) is a 71.721 kD protein (640aa) expressed in cholinergic neurons of both the PNS and CNS. In the CNS, ChAT is expressed in motor neurons and pre-ganglionic autonomic neurons of the spinal cord, a subset of neurons in the neostriatum, and in the basal forebrain. In the PNS, ChAT is expressed in a small subpopulation of sympathetic neurons and in all parasympathetic neurons. ChAT is the enzyme responsible for synthesis of acetylcholine from acetyl-coenzyme A and choline.

Applications:
Suitable for use in Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:1000 -1:2000
Immunocytochemistry: 1:1000 -1:2000
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1906 IgY, Chicken Pab Rb x Ch
I1906-10 IgY, Chicken (HRP) Rb x Ch
I1906-12 IgY, Chicken (FITC) Rb x Ch
I1906-14 IgY, Chicken (AP) Rb x Ch

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgYAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseChicken
Concentration:
~0.1mg/ml
Immunogen:
Synthetic peptide corresponding to Choline Acetyltransferase (KLH). Species sequence homology: human (P28329, NCBI) and mouse (Q03059, NCBI) 100%.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity:
Recognizes Choline Acetyltransferase. Species crossreactivity: mouse. Species sequence homology: human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.