Technical Data
C5057-06
Choline Acetyltransferase (CHAT)
Description:
Acetylcholine (Ach) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransfrase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-cholinacetyltransferase a useful specific marker.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1:1000-1:5000
Immunohistochemistry: 1:500-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul-20CBlue IceHumanGoat
Concentration:
Not determined
Immunogen:
Human placental enzyme.
Purity:
Serum
Form
Supplied as a liquid with no preservatives added.
Specificity:
Recognizes cholinergic neurons in human brain and central nervous systems. Species Crossreactivity: Mouse, guinea pig and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Brain Res. Bull. 18: 447455 (1987). 2. Sleep 11: 116 (1988). 3. Neuroscience 32: 685700 (1989). 4. Brain Res. 517: 224228 (1990). 5. Brain Res. 532: 317322 (1990). 6. J. Neurosci. Methods 35: 3137 (1990). 7. Abstr. Soc. Neurosci. 18: 1483, #624.2 (1992). 8. Brain Res. Bull. 25: 129137 (1990). 9. Brain Res. Bull. 29: 635642 (1992). 10. Neurosci. Lett. 147: 217220 (1992). 11. Brain Res. 627: 4954 (1993). 12. Neurobiol. Aging 14: 389392 (1993). 13. Cell 75: 12731286 (1993). 14. Cell 75: 11991214 (1993). 15. Neuroscience 54(4): 845857 (1993). 16. Exp. Neurol. 130: 230236 (1994). 17. J. Comp. Neurol. 342: 497537 (1994). 18. J. Neurochem. 65: 484491 (1995). 19. Neuron 14: 717730 (1995). 20. Exp. Neurol. 131: 239250 (1995). 21. J. Neurosci. 17: 76447654 (1997). 22. Exp. Neurol. 144: 350360 (1997). 23. J. Comp. Neurol. 382: 499534 (1997). 24. J. Neurosci. 22: 7680-7686 (2002). 25. Brain Res. 608(2): 223-232 (1993).