Technical Data
C5057-06A
Choline Acetyltransferase (CHAT, CMS1A, CMS1A2, choline acetyltransferase, acetyl CoA:choline O-acetyltransferase)
Description:
Alternate Name: CHAT, CMS1A, CMS1A2, choline acetyltransferase, acetyl CoA:choline O-acetyltransferase

Domain Information:

Gene Ontology Terms:

Application(s):
Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 70kD band observed in Human Placenta lysates (calculated MW of 70.5kD according to NP_065574 and NP_066264). Recommended concentration: 0.03-0.3ug/ml.

0.03ug/ml staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Peptide Blocking:
Corresponding peptide is available for Peptide Blocking studies. See C5057-06A-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
CKEKATRPSQGHQP, C Terminus
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity:
This antibody is expected to recognize both reported isoforms. NP_066264, NP_066265 and NP_066266 are identical proteins representing isoform 1. Species Crossreactivity: Human, chimpanzee, porcine, canine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Madziar B, Lopez-Coviella I, Zemelko V, Berse B. , Regulation of cholinergic gene expression by nerve growth factor depends on the phosphatidylinositol-3'-kinase pathway., J Neurochem. 2005 Feb;92(4):767-79.