Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|100ul||4°C (-20°C Glycerol)||Blue Ice||Rat||Rabbit|
| Not determined.|
|A 22 amino acid synthetic peptide from porcine ChAT (KLH coupled). Peptide sequence H-GLFSSYRLPGHTQDTLVAQKSS-NH2.|
|Supplied as a lyophilized powder. Reconsitute in 0.1ml sterile dH2O or 40-50% glycerol for long-term storage.|
|Will stain cholinergic neurons in rat central and peripheral nervous systems. Species Crossreactivity: Rat. Other antibodies produced against the same peptide have shown to react against primate and human enzyme.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Benecke et al. (1993) J. Neurochem 61: 804–811.|