Technical Data
Choline Acetyltransferase (CHAT)
Acetylcholine (Ach) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransfrase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-cholinacetyltransferase a useful specific marker.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: 1:2000-1:4000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ul-20CBlue IceRatRabbit
Not Determined
A synthetic peptide corresponding to 22aa H-GLFSSYRLPGHTQDTLVAQKSS from porcine ChAT, conjugated to KLH
Supplied as a lyophilized powder. Reconsitute with 100ul sterile ddH2O.
Recognizes rat choline Acetyltransferase. Will stain cholinergic neurons in rat central and peripheral nervous systems. Other antibodies produced against the same peptide have shown to react against primate and human enzyme.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Benecke et al. (1993) J. Neurochem 61: 804811.