Technical Data
C5057-09
Choline Acetyltransferase (CHAT)
Description:
Acetylcholine (Ach) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransfrase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-cholinacetyltransferase a useful specific marker.

Applications:
Suitable for use in Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: 1:100-1:250
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG12Q28Ascites
SizeStorageShippingSourceHost
100ul-20CBlue IceRatMouse
Concentration:
Not Determined
Immunogen:
Choline acetyltransferase purified from rat brain
Purity:
Ascites
Form
Supplied as a liquid.
Specificity:
Recognizes rat cholinergic neurons in the brain and central nervous system. Reported to crossreact with chicken.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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