Technical Data
C5062-75A
Cholinergic Receptor, Nicotinic, beta 2 (CHRNB2, EFNL3, nAChRB2, cholinergic receptor, nicotinic, beta Polypeptide 2 (neuronal), neuronal nicotinic acetylcholine receptor beta 2)
Description:
Neuronal acetylcholine receptors are homo- or heteropentameric complexes composed of homologous alpha and beta subunits. They belong to a superfamily of ligand-gated ion channels which allow the flow of sodium and potassium across the plasma membrane in response to ligands such as acetylcholine and nicotine. This gene encodes one of several beta subunits. Mutations in this gene are associated with autosomal dominant nocturnal frontal lobe epilepsy.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:64000
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human Brain (Cerebellum, Cerebral Cortex) lysates

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide with sequence QPRHHCARQRLR, from the internal region of human CHRNB2 (NP_000739.1).
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in Tris, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes human CHRNB2 at ~48kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Cook LJ, Ho LW, Taylor AE, Brayne C, Evans JG, Xuereb J, Cairns NJ, Pritchard A, Lemmon H, Mann D, St Clair D, Turic D, Hollingworth P, Moore PJ, Jehu L, Archer N, Walter S, Foy C, Edmondson A, Powell J, Lovestone S, Owen MJ, Williams J, Lendon C, Rubinsztein DC. Candidate gene association studies of the alpha 4 (CHRNA4) and beta 2 (CHRNB2) neuronal nicotinic acetylcholine receptor subunit genes in Alzheimer's disease. Neurosci Lett. 2004 Mar 25;358(2):142-6. PMID: 15026168